Gebrehiwot Tadesse
Mekelle University
Ethiopia
Title: Alternatives for inhibition of methanogenesis and enhance fermentation of feeds in the rumen
Biography
Biography: Gebrehiwot Tadesse
Abstract
The aim of the present study was to investigate methanogenesis and fermentation pattern on wheat straw based diet with extracts of Albizialebbeck leaves and supplementation of corresponding levels of leaves on rumen fermentation profile in buffaloes. Extracts (100 ml) were prepared using three solvents, methanol, petroleum ether and water, with 10 g powdered sample; and stored at 4 C for subsequent use. The extracts were tested at three levels (0, 0.25 and 0.50 ml). A mixture (200 ± 5 mg) of wheat straw and concentrate mixture (1:1) was used as substrate and incubated with 30 ml buffered rumen fluid in 100 ml calibrated glass syringes at 39 C for 24 h following standard IVGP protocol. The total gas and methane production was reduced (p< 0.05) with the inclusion of extracts, irrespective of solvent. However, the reduction in ruminal methane production was more pronounced with the methanol and aqueous extracts of Albizialebbeck leaves. Feed degradability was reduced (p<0.05) with petroleum ether extract, however, aqueous extract did not exert any negative effect. Microbial protein synthesis, partitioning factor and propionate production was improved (p<0.05) with the inclusion of methanol and aqueous extracts. Further, an experiment on in vivo rumen fermentation profile was conducted on three fistulatedMurrah buffalo steers supplemented with fresh Albizia leaves at three levels (0, 300g, and 600g per day) in 3x3 Latin Square Design. Supplementation (300g/animal/d) of Albizia leaves to fistulated buffaloes resulted in increase (p<0.05) in total volatile fatty acids and propionate production; however, higher level (600g/animal/day) of supplementation exerted negative effects on rumen fermentation. Increase in total-N as well as ammonia-N concentration was also observed due to ALB-1 supplementation. It may be concluded that Albizialebbeck leaves (300g/animal/day) have a potential to modulate rumen fermentation towards reduced methanogenesis and enhanced volatile fatty acids production